Hi, Everyone, this is China Care Medical, today let us introduce some basic knowledget about Covid19 Detection Methods.
Part 1,
Three test principle test method introduce.
1, PCR based Nucleic Acid Test
Advantage: High Accurancy, Accepted by authoritative certification worldwide, can be used for official report to take plane or boat, or move from one place to another place.
And it is most profitable project for hospitals or clinic, the consumable cost of one test is around 10 USD, but the test cost for one people is more than 50 to 100 USD.
Disadvantage: High cost for the full set of equipment, some small labroom or clinic can't afford to start it, and it requests more strict test environment, compare with the other test principle, it cost more time to finish one test( around 3 hours, other menthods only need around 15 to 25 Munites.
(PCR Nucleic Acid Test Process)
2,Antigen Test, test the presence of the viral proteins and can return postive result when a person is most infectin.
There are two ways for Antigen Test principle,
One, use One Step Test for Antigen, this is by Colloidal Gold, this way was common usage by the general test for many quantity people.
Advantage: Cheap cost, only test is around 1.5 to 2 USD, and no need extra equipment to support, and get result quickly.
Disadvantage: Can't detect the detail value of Antigen of one person, can only result for the Negative and positive, and the result is easy to influence by other condition, after testing by this method, it still need to test by PCR or other method to confirm the result.
(Antigen Test-One stop Test Kit, Click Above picture to get price)
Two, Immunofluorescence Assay principle to test Antigen, By this way, the covid quantity can be marked by Immunofluorescence material, then test by one machine and report the value.
Advantage: High Accurancy, and affordable cost for the machine and consumable, it can help many small labrooms or Clinic start to offer this service, it can get result quickly only 15 to 20 Munites.
Disadvantage: Some countries is not accepted this mentioned as the official test way, maybe not use for report to take plane or others.
(Antigen Immunofluorescence Test Kit, Click Above picture to get price)
3,Antibody Test, Detect the body's immune response to the virus.
There are two ways for Antigen Test principle,
One, use One Step Test for Antibody, this is by Colloidal Gold, this way was common usage by the general test for many quantity people.
Advantage: Cheap cost, only test is around 1.5 to 2 USD, and no need extra equipment to support, and get result quickly.
Disadvantage: Can't detect the detail value of Antigen of one person, can only result for the Negative and positive, and the result is easy to influence by other condition, after testing by this method, it still need to test by PCR or other method to confirm the result.
(Antibody Test-One stop Test Kit, Click Above picture to get price)
Two, Immunofluorescence Assay principle to test Antibody, By this way, the covid quantity can be marked by Immunofluorescence material, then test by one machine and report the value.
Advantage: High Accurancy, and affordable cost for the machine and consumable, it can help many small labrooms or Clinic start to offer this service, it can get result quickly only 15 to 20 Munites.
Disadvantage: Some countries is not accepted this mentioned as the official test way, maybe not use for report to take plane or others. And the Antibody produced after several days later when person infected, so during that period, this way maybe not test it out.
(Antibody Immunofluorescence Test Kit Picutre, Click Above picture to get price)
The Probability of different methods,
Part 2
The Equipment List for each Nucleic Acid Test,
PCR based Nucleic Acid Test, According to the whole process, it can be summarize for three steps,
Step 1: Sample Collecting and Preservation,
Step 2: RNA Extraction
Step 2: PCR machine process
According to above three process, we suggest you prepare at least two or three rooms separetely.
Room 1, Sample Room for Sample Collecting and Preservation,
Doctors should wear the protect clothes and use sampling tube with nasal and throat swab to get virus sample from patient.
Material List,
1, Doctor protect clothes.
(Medical Protect Clothes Picutre, Click above picture to get price of protect clothes)
2, Disposable nasal and throat swab to get sample and virus sampling tube to storage sample.
(Disposable nasal and throat swab and tubes Picutre, Click above picture to get price of nasal and throat swab and virus tubes)
3, Medical Freezer to storage sample at 2-8 degrees.
(Medical Freezer Picutre, Click above picture to get price of Medical Freezer)
Room 2, Nucleic acid extraction Room,
In this room, we can get the Nucleic acid by hand or by machine.
Material List,
1, Freezer to store the reagent of Nucleic acid extraction.
(Medical Freezer Picutre, Click above picture to get price of Medical Freezer)
2, Biochemistry Cabinet, to avoid the room environment Contaminated by the Nucleic acid process, protect better for the test staff to be infected. About Biochemistry analyzer, it has different Class, includes Class I, Class II and others, From Class I to Class II, the cost is increase. we suggest the Class II is basic lowest grade for Covid 19 test,
Some big hospitals will have the professional Negative pressure room, this is the best conditions for Nucleic acid extraction test, but if you don't have enough time and money to get Negative pressure room, one Biochemistry Cabinet can be also ok.
(Biochemistry Cabinet Picutre, Click above picture to get price of Biochemistry Cabinet)
3, Nucleic acid extraction Reagent,
Nucleic acid extraction Hand operation Kit, it is cheap way, but need to cost some time to operate it.
(Nucleic acid extraction Reagent Picutre, Click above picture to get price of Nucleic acid extraction Reagent)
Nucleic acid extraction Automated machine, it can save a lot of time, but the machine is little expensive, if you are interested in the machine, please inquiry us.
4, Centrifuge,
(Centrifuge Picutre, Click above picture to get price of Centrifuge)
5, Pipette,
(Pipette Picutre, Click above picture to get price of Pipette)
6, Pipette tips, It must Rnase & Dnase free,
(Pipette tips Picutre, Click above picture to get price of Pipette Tips)
Room 3, Gene amplification room for PCR machine process.
In this room, we will apply for the Gene amplification process by the Nucleic acid extracted by Room 2, and get result for the test.
Material List,
1, Real time PCR Machine
(Real Time PCR Picutre, Click above picture to get price of Real Time PCR )
2, Real time PCR Machine Reagent
(Real Time PCR Reagent Picutre, Click above picture to get price of Real Time PCR Reagent )
3, Pipette and Tips,
Check Room 2 list to find price for Pipette and Tips
4, Ultralow Temperature Refrigerator, to storage PCR reagent is requested -25°C to -40°C temperature.
(Ultralow Temperature Refrigerator Picutre, Click above picture to get price of Ultralow Temperature Refrigerator)
The Equipment List for other test method,
Please Check Part 1 introduce.